Identification and differentiation of Candida parapsilosis complex species by use of exon-primed intron-crossing PCR.

نویسندگان

  • Xiaobo Feng
  • Zengbin Wu
  • Bo Ling
  • Shuming Pan
  • Wanqing Liao
  • Weihua Pan
  • Zhirong Yao
چکیده

The Candida parapsilosis complex is composed of Candida parapsilosis sensu stricto, Candida orthopsilosis, Candida metapsilosis, and the closely related species Lodderomyces elongisporus. An exon-primed intron-crossing PCR assay was developed here to distinguish the members of the species complex on the basis of the distinct sizes of amplicons, and Candida orthopsilosis and Candida metapsilosis were further discriminated by restriction enzyme analysis.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 52 5  شماره 

صفحات  -

تاریخ انتشار 2014